Characterization of flavone synthase I from rice

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Characterization of flavone synthase I from rice.

Flavones are synthesized from flavanones through the action of flavone synthases (FNSs). There are two FNSs, FNS I and II. FNS I is a soluble dioxygenase present in members of the Apiaceae family and FNS II is a membrane bound cytochrome P450 enzyme that has been identified in numerous plant species. In this study, we cloned OsFNS I-1 from rice by RTPCR, expressed it in E. coli, and purified th...

متن کامل

Cloning of parsley flavone synthase I.

A cDNA encoding flavone synthase I was amplified by RT-PCR from leaflets of Petroselinum crispum cv. Italian Giant seedlings and functionally expressed in yeast cells. The identity of the recombinant, 2-oxoglutarate-dependent enzyme was verified in assays converting (2S)-naringenin to apigenin.

متن کامل

Expression of parsley flavone synthase I establishes the flavone biosynthetic pathway in Arabidopsis thaliana.

Arabidopsis thaliana lacks the flavone biosynthetic pathway, probably because of a lack or low activity of a flavone synthase. To establish this biosynthetic pathway in Arabidopsis, we subjected this model plant to transformation with the parsley gene for flavone synthase type I (FNS-I). Transgenic seedlings expressing FNS-I were cultured in liquid medium with or without naringenin, and plant e...

متن کامل

Induction and Characterization of a NADPH-Dependent Flavone Synthase from Cell Cultures of Soybean

Microsomal preparations from osmotically stressed soybean cells catalyze the conversion of (25)-naringenin to apigenin in presence of N A D P H . In contrast, such preparations from normal soybean cells or from elicitor-challenged cells catalyze the conversion o f (25)-naringenin to genistein (isoflavone synthase). It is concluded that osm otic stress of the cells causes a switch from isoflavon...

متن کامل

Function and characterization of starch synthase I using mutants in rice.

Four starch synthase I (SSI)-deficient rice (Oryza sativa) mutant lines were generated using retrotransposon Tos17 insertion. The mutants exhibited different levels of SSI activities and produced significantly lower amounts of SSI protein ranging from 0% to 20% of the wild type. The mutant endosperm amylopectin showed a decrease in chains with degree of polymerization (DP) 8 to 12 and an increa...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BMB Reports

سال: 2008

ISSN: 1976-6696

DOI: 10.5483/bmbrep.2008.41.1.068